AP Biology Lab 1: Diffusion & Osmosis Activity 1b: Osmosis.I have concluded that my hypothesis was correct, since the date reinforces that the mass is greater after being put into the beakers of distilled water.The independent variables in this lab are the beakers of distilled water, and the amount of sucrose (0.2-1.0M).My hypothesis is: if the dialysis tubing that is filled with sucrose solution and fully emerged in a beaker of distilled water, then the water will seep into the dialysis tubing and the tubing will become a greater mass.Osmosis has occurred, because the water molecules have diffused into the dialysis tubing.
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In an isotonic solution, there is no net movement and there is an equal concentration of solutes and water.Water goes through the cell membranes by diffusion.Osmosis is specifically the movement of water through membranes.We observed different solutes (NaCl, Ovalbumin, Glucose, Sucrose, and Water) in the dialysis tubing.It is important for an IV solution to have salts in it so the water and solute can be equal to create an isotonic environment.
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5 drops of water was also added to a well, functioning as a control.All the test tubes were then placed in a hot water bath using the hot plate.AP Biology Formal Lab Results of September 12.AP Biology Class Notes of Macromolecules.AP Biology Identification of Carbohydrates Lab Instructions of September 12.
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The first step of this experiment was to fill one tub with room temperature water and another with ice water and put a thermometer in each.Table 1 shows the amount of oxygen consumed by both types of peas in both the room temperature water and the ice water.Water | Unsprouted Peas: Ice Water | Glass Beads: Room Temp.Temp| Green Lettuce: Ice Water| Iceberg Lettuce: Room Temp| Iceberg Lettuce: Ice Water| 0 minutes (Equilibrium)| .36| 1.2| .21| .95| .32| .92| 5 minutes| .38| 1.5| .23| 1.03| .36| 1.05| .Later we filled one tub with room temperature water and another with ice water and then put thermometers in each.
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This occurred because the tubings were placed in a beaker of water.This means that there was a higher concentration of water than the solute, causing the water to diffuse in the cell after 30 minutes.The hypertonic solution has a higher solute concentration and lower water potential.The five cells were placed in a beaker filled with water for 30 minutes.Hypotonic is when the solution has a lower solute concentration compared to the water potential.
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According to AP Lab #6: pGLO Transformation Lab, it states that the –pGLO bacteria that didn’t have the plasmid couldn’t survive on the ampicillin plates, which eventually resulted in no bacterial growth.“AP Lab #6: PGLO Transformation Lab.” .The findings of this lab experiment are very important, because genetic transformation doesn’t only involve making bacteria glow in UV light, but it involves a lot more such as, making fish glow under fluorescent light, or making crops resistant to air borne diseases.In this lab experiment there were four different outcomes.In this lab, there might have been some sources of errors that could have affected the results of this experiment.
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Please Refer to Biol 368 lab manual* .Material and Methods: .Then, site specific recombination would not occur.For this to be inserted to the cell, lambda phage was used.Tn10 would be successfully inserted by lambda phage and create random mutations.
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Begin by setting up a 10°C bath to allow time for the temperature of the water to adjust.Then place the first three vials into the 10°C water bath.Moving the vials and putting your hands in the water could have caused contamination.Place a sheet of paper underneath to make the amount of water entering the pipette easier to see and measure.After seven minutes, submerge the three respirometers into the baths, covering them complete with water.
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Lastly, to rid the lab of any weaknesses, the procedure would have to be changed to access and outline the wide array of variables that must be controlled to assure the validity of the data.By boiling water in one part of the experiment, we were able to see the effects of temperature on reaction rate.Having a lack of control variables, the lab remains subject to the countless ever-changing variables.Water and Oxygen .In following the procedure outlined in the AP lab manual, there were no problems stemming from the procedure itself that would have potentially challenged the results gathered.
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Potatoes must contain sucrose molecules due to the conclusion of this lab because the potatoes take in water in the distilled water beaker.Water potential is affected by two factors: osmotic potential (Ψπ) and pressure potential (Ψp).Exercise 2 showed that the potato samples took in water when immersed in a distilled water solution.The equation to find the sum of water potential is: Water Potential = Pressure Potential + Osmotic Potential .Osmotic potential is dependent on the solute concentration, and pressure potential which is the energy that forms from exertion of pressure either positive or negative on a solution.
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.. MTT assay performed on MCF-7 human breast cancer cell line for anticancer potential of CNDs with 5-Fluorouracil as control drug shows moderate to good activity against these cells.So these water soluble CNDs derived from natural herb and in basic medium by carbonization process can be used as tagging and coating material on bioactive nanomaterials for cell vectorization or probing and for biocatalytic, antioxidant and anticancer applications.The double distilled water from Millipore system was used throughout the synthesis and testing.As formed carbon residue was diluted with double distilled water to 100 ml.water taken in cuvette and 1 ml.
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Determining the Water Potential of Potato Tubers using Gravimetric and Chardakov Techniques 1.In this experiment The Gravimetric and Chardakov techniques are used for determining the W for potato tubers by incubation in inc... ... middle of paper ... ...of the water potential of stored potato-tubers.Measuring the Water status of Potato tubers.St. John’s University, Minnesota.Another factor affecting W is the pressure potential (P) which could be either positive for turgor cells or zero for flaccid cells or negative for cells with active transpiration.
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In addition, I am involved in aquatic sports such as water polo and the swim team whose practices occupy most of my schedule and energy.With some self-control, one can direct himself back to a world of tranquil simplicity by slowing down and enjoying and appreciating the simple things that once filled our lives with joy.There is a remedy, and a simple one at that.I am frequently awake at midnight or later studying for an upcoming biology test or completing a chemistry lab.For example, I am currently enrolled in two science classes: AP Biology and Chemistry.
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Carrot 1 had gone through hypotonic osmosis, where the water goes through the cell membrane.Discussion: All three of these labs worked out, and ended pretty well except our second lab, the carrot lab.We then put Carrot 1 into the water, while Carrot B went into the salted water.But that had made no sense to us because the carrot that was in the salt water should’ve shrunk and not gained weight.We knew that the glucose from the dialysis bag, had come out and into the beaker, and we knew that it had to be through diffusion because it wasn’t just water in the beaker.
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As a student who took two AP classes during high school I felt it was a struggle to keep up with my other classes because I was too focused on my AP courses.The pilot program began running in 1952.The College Board, a non-profit organization, has run the AP program since 1955.The courses for AP vary from physics, to chemistry, to biology, all the way to history, geography, and english.If a student takes enough AP classes and gets a good score on the AP exam, it is possible to graduate from college a semester or even a year early.If the course is approved, the school may use the AP title and the course will be publicly listed on the AP course list.Stanley-Becker’s column had incredible and passionate responses, including one from a high s...
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On the contrary, if solute is added to the beaker, the water potential in the cell will be greater, resulting in a hypotonic cell.The water moves from a place of higher water concentration to a place of lower water concentration.Therefore the water will move into the cell because water moves from hypotonic environments to hypertonic environments.Water potential measures free energy of water in any solution.Water potential of distilled water in atmospheric pressure is 0, because the osmotic and pressure potentials are 0.
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Every enzyme has different characteristics and by conducting this specific lab with a different enzyme, the characteristics can be determined.These two potential experiments for the lab based on the factors that affect enzyme activity can be used to further understand the concept of enzyme activity.By learning about enzymes in class, as well as conducting this specific lab, the knowledge about biological catalysts was extended and can be connected to situations that occur daily.Thus, catalase battles against the effect of these molecules by transforming them into hydrogen peroxide, and then later breaking it into oxygen and water (eHow).The substrate in this lab that was used was hydrogen peroxide, the substance that is provided from the...
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(10-17.438)^2/17.438 = 3.172 .All their food and water needs are taken care of by the substance called media in the bottom of the vial.Table 3: Lab Group Data for Cross 2 .. Chi Square Analysis for Lab Group Data for Cross 2 .Table 1: Lab Group data for Cross 1 .. Chi Square Analysis for Lab Group Data for Cross 1 .(2-5.813)^2/(5.813) = 2.501 .
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Cheating has become too easy to justify.The means are everywhere, the perpetrators are everyone, and the results are guaranteed.Too much pressure to be talented, and service-oriented, and diverse, but dedicated.It’s a frightening statement, but I think that the ends really have come to justify the means.Even Jonah Bartrand, who is in all respects a shining example of academic purity, admitted the next day that he had collaborated on lab write-ups, which his biology teacher clearly forbade.
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Lab 6.4 Review .Why or why not?Radio signals attenuate through walls and obstacles.How would this affect AP placement in a home or office?A highly directional antenna has a dispersion of 80 degrees to as little as 28 degrees, and is used for point-to-point transmission.
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There is no literature available for anticataract activity of AP and LC.Gupta SK, Kalaiselvan V, Srivastava S, Agrawal SS, Saxena R (2010): Evaluation of anticataract potential of Triphala in selenite-induced cataract: In vitro and in vivo studies.APE and LCE group had significantly higher concentrations of total lens proteins and water soluble proteins (P<0.01), compared to toxic control group (Fig-1).In conclusion, the study demonstrated that monotherapy of AP and LC had potential to prevent cataract due to their strong antioxidant potential.Conclusion: The study demonstrated that monotherapy of APE and LCE respectively, has the potential to prevent cataract because of the strong antioxidant potential of each.
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When a hydrophobic group enters the body of water, the hydrogen bonds in the water break apart, yet cannot bind to the r group on the amino acids, so the water forms bonds with itself again around the r-group, thus pushing the r-group away due to the magnetic forces that push similarly charged atoms away from eachother.Benthic: Lives one the bottom of a body of water .To do so, our lab group first accessed online research websites to compare the phenotypes of the different Ichthyoids.The purpose of this lab is to study the physical attributes and proteomics of different species of fish to determine the potential genealogical tree connecting these species .We would use this information to calculate the weight of these bands by comparing t...
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Dehydrated: high solute concentration in body due to lack of water consumption, water will come out of cell into bloodstream to fix it You don’t give dehydrated person water quickly at once, you can cause water to rush into cell and rupture RBC’s Osmolarity: the total concentration of solute particles in a solution (the type of particle doesn’t matter Ex.1 Propogation of Action Potential AP must be transmitted to next neuron, depolarization of adjacent membranes away from origin Unidirectional because area where AP was just generated has inactivated Na+ gates.Wherever salt goes, water follows), water goes to wherever there is more solute Ex.3 Osmosis Definition: unassisted diffusion of water from area of low to one of high solute concent...
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& Carolina Student Guide for AP Biology Laboratory 4: Plant Pigments and Photosynthesis) Background Information (Activity B): .Use the same pipet to add 3 mL of distilled water to cuvettes 2-5.* Heat sink (aquarium filled with water) .Use a new, clean dropping pipet to add 4 mL of distilled water (H2O) to Cuvette 1.In this part of the lab, we will add a solution of DPIP, which is a blue dye to a suspension of chloroplasts, the plant cell organelle that conducts photosynthesis.
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If this lab was done over again, it would most likely be done on a larger scale.Overall, the lab was very successful.From this lab, there were no errors.See science manual Bacterial Transformation Lab for complete list of materials and procedures.All in all, the lab was fun and a success in transforming the bacteria, e-coli, into one that is antibiotic resistant and into one that can glow.
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This could have also been possible with the inhibitor part of the lab.“Enzyme Lab – Ex.Amount of Distilled Water (mL) ... One other suggestion would be to carry out the experiments to the full potential.Catalase breaks down hydrogen peroxide into water and oxygen.
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This lab research is to study the functional attributes of living enzymes, employing a quantitative approach to their measurement.Mussel volume to ascertain the exact volume of water in the chamber.The lab deals with the examination of gill area, shell volume & foot weight scale with the size of mussels; observing how the size of the mussel effects the different biological processes.The objective of the study conducted was to find out the effects of respiration, water pumping activity & environmental stresses on the mussel’s growth.Leave for 15 minutes then measure the absorbance against 750nm distilled water.
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To avoid this make sure apparatus is in the middle of a flat bench and make sure if it uses water to connect it properly so avoid leaks, in the case of a spillage simply mop up using paper towels, if the slipping occurs then if injured get a first aid officer to check you.Although some standards are area-specific, there will often be substantial overlap across areas and this is considered during building of the standard.In the case of the substance being in contact with the skin wash off quickly and put the area affected in ice or cold water.It can include items such as safety gloves, eye protection such as goggles, visors and glasses, safety footwear and lab coat.Where appropriate referrals are combined and developed as a combined quali...
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As a result, it was difficult to observe the effect of water volume on the rate of osmosis.On the other hand, a hypotonic solution outside the cell will cause water to enter the cell, and if the cell takes up too much water, it may lyse, or burst.For example, reverse osmosis, which relies on not only the concentration gradient, but also the pressure gradient and water flux, is used for purification and wastewater treatment (Bodalo-Santoyo 101).Therefore, it made sense that the water molecules exited the bags in order to dilute the more highly concentrated sucrose-water solutions in the beaker.I hypothesize that a larger volume of water in the dialysis tubing translates to a faster osmosis rate as well due to increased pressure.
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Inside the gills of aquatic animals, the circulatory system removes oxygen, and delivers waste carbon dioxide.Maybe you would like to answer that?Aquatic animals use gills, which are outgrowths from the body which increase surface area over which gas exchange can occur.Alveoli are sack-like structures where the circulatory system meets the respiratory system.For each problem, explain a physiological of structural solution.
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