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“Evaluation of in-vitro anti-cataract activity of Abies pindrow & Luffa cylindrica”: A Comparative study . Suchita Dubey, Sudipta Saha, Shubhini A Saraf* . Context- Cataract is the opacification of lenses resulting in blurring of vision.Oxidative stress is the major cause of many diseases including cataract. Objective- The study was designed to evaluate and compare the in-vitro anti-cataract activity of aqueous extracts of Abiespindrowleaves (APE) and Luffacylindrica fruits (LCE) against hydrogen peroxide induced cataractogenesis, using isolated goat lenses. Materials and Methods-Standardized extracts of APE and LCE were compared for their anti-cataract activity against marketed eye drops.
...Middle of the Anti-cataract Activity of Abies Pindrow & Luffa Cylindrica...
Group 12-PSS + Ab 250 mg (1%) + LCE (25%) . Group 13-PSS + Ab 250 mg (1%) + LCE (30%) . Homogenate preparation- After incubation, lenses were homogenized in 10 volumes of 0.1M potassium phosphate buffer, pH 7.0. The homogenate was centrifuged at 10,000 rpm for 1 hour and the supernatant was used for estimation of biochemical parameters. Biochemical estimation- SOD and GSH levels were measured using Ellman’s method.
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(1959) Tissue sulfhydryl groups. Arch Biochem Biophys. Lowry OH., Rosenberg NJ., Farr AL., Randall RJ (1951) Protein measurement with the Folin phenol reagent. Bar-Or D., Rael LT., Lau EP., Rao NK., Thomas GW.,Winkler JV., Yukl RL., Kingston RG. (2001) An analog of the human albumin N-terminus (Asp-Ala-His-Lys) prevents formation of copper-induced reactive oxygen species.
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GPx activity has been reduced in selenium deficiency (Muller et al., 1984; Son et al., 2007). It was shown that weakening of GSH enhances cerebral ischemic injury in rats (Mizui et al., 1992; Son et al., 2007).
For example, liver injury included by consuming alcohol or by taking drugs like Paracetamol, lung injury by smoking and muscle injury by intense physical activity, all are known to be correlated with low tissue level of GSH. The observed increase of SOD, CAT and GSH activity in liver suggests that the LIALC extract have an efficient protective mecha...
Activity measurement (U / L) = [(Δ150sn-Δ30sn) / 2] x1202.84 . A wide variety of oxidizing molecules such as ROS and/or depleting agents can change the glutathione redox state, which is normally maintained by the activity of GSH depleting (GSH-Px) and replenishing enzymes (glutathione reductase).
Y – Absorbance of test sample . The colour developed was read at 412 nm.
.. Peroxidase activity was measured spectrophotometrically by following the change in absorbance at 460 nm due to o-dianisidine oxidation in the presence of H2O2 and enzyme. .. Glutathione peroxidase activity was measured by the method described by Paglia and Valentine (1967).
The study shows that hydrolysis of glycosides accelerates their absorption in the small intestine .it is site where metabolic conversion takes place because of enzymatic activity of sulfation and glucuronation. The study suggested that quercetin showed its neuroprotective effects on colchincine induced cognitive dysfunctions by significantly changin...
Differences between the groups were considered significant at pË‚0.05 . The catalase activity of the hemolysate is determined by adopting the method of Brannan et.al.
The authors would like to express their sincere thanks and gratitude to the study subjects and controls for their participation. From the results it is evident that consumption of 90ml of alcohol significantly increases the activities of enzymatic antioxidants on comparison with control participants and those consuming 120ml of alcohol.
Glutathione transferase activity using 2, 4 dichloronitrobenzene as substrates was assayed spectrophotometrically by Habig . Glutathione Peroxidase activity was assayed according to the procedure of Rotruck .
JGT showed higher reducing power and DPPH radical scavenging activity. JGT showed the highest metal chelating activity percentage (76.38%) followed by DGT(73.62%) among regular green teas.
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