Anti-cataract Activity of Abies Pindrow & Luffa Cylindrica

...Start of the Anti-cataract Activity of Abies Pindrow & Luffa Cylindrica...

“Evaluation of in-vitro anti-cataract activity of Abies pindrow & Luffa cylindrica”: A Comparative study . Suchita Dubey, Sudipta Saha, Shubhini A Saraf* . Context- Cataract is the opacification of lenses resulting in blurring of vision.Oxidative stress is the major cause of many diseases including cataract. Objective- The study was designed to evaluate and compare the in-vitro anti-cataract activity of aqueous extracts of Abiespindrowleaves (APE) and Luffacylindrica fruits (LCE) against hydrogen peroxide induced cataractogenesis, using isolated goat lenses. Materials and Methods-Standardized extracts of APE and LCE were compared for their anti-cataract activity against marketed eye drops.

...Middle of the Anti-cataract Activity of Abies Pindrow & Luffa Cylindrica...

Group 12-PSS + Ab 250 mg (1%) + LCE (25%) . Group 13-PSS + Ab 250 mg (1%) + LCE (30%) . Homogenate preparation- After incubation, lenses were homogenized in 10 volumes of 0.1M potassium phosphate buffer, pH 7.0. The homogenate was centrifuged at 10,000 rpm for 1 hour and the supernatant was used for estimation of biochemical parameters. Biochemical estimation- SOD and GSH levels were measured using Ellman’s method[6].

...End of the Anti-cataract Activity of Abies Pindrow & Luffa Cylindrica...

(1959) Tissue sulfhydryl groups. Arch Biochem Biophys. Lowry OH., Rosenberg NJ., Farr AL., Randall RJ (1951) Protein measurement with the Folin phenol reagent. Bar-Or D., Rael LT., Lau EP., Rao NK., Thomas GW.,Winkler JV., Yukl RL., Kingston RG. (2001) An analog of the human albumin N-terminus (Asp-Ala-His-Lys) prevents formation of copper-induced reactive oxygen species.

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