Classification of Macromolecules


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The purpose of this lab was to determine if various substances contained macromolecules, specifically; carbohydrates, proteins, or vitamin C. The tests used were the Biuret test, the xanthoproteic test, the Benedict’s test, the starch test, and the indophenol test. Many of the substances were positive for that which they were being tested, proving the hypothesis partially correct. INTRODUCTIONThis lab was conducted to determine if various substances contained various macromolecules. The macromolecules tested for were carbohydrates, proteins, and vitamin C. The substances tested were glucose, fructose, sucrose, maltose, milk, corn syrup, starch, glycogen, gelatin, egg albumin, ascorbic acid, apple juice, orange juice, and fruit punch.


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A color changed indicated a positive result. The results from the first two tests were then recorded in a table. (Malyk, 2007b)Tests for protein were conducted next, and the substances used were egg albumin, gelatin, and milk. These substances were tested using the Biuret test and the xanthoproteic test. The Biuret test was conducted by added 2 mL of each solution to a test tube, along with a distilled water control.


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“Carbohydrates”. Michigan State University. (2007a) http://www.cem.msu.edu/~reusch/VirtualText/carbhyd.htm12. Michigan State University. (2007b) http://www.cem.msu.edu/~reusch/VirtualText/proteins.htm .


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