Mutation In E Coli Bacterial Cells

...Start of the Mutation In E Coli Bacterial Cells...

In this experiment, mutation in E. coli bacterial cells is learned. Mutations occur when DNA sequence are altered: either deletions or insertions of a single or multiple base pair that can lead to frameshift mutations. Mutation frequency measures how frequently the cells are mutated in a given population.

...Middle of the Mutation In E Coli Bacterial Cells...

Afterwards, it can be tested by screening with IPTG and X-gal containing plate where it would show blue when Lac Z is active. However, this screening method is not used in this experiment. Instead, replica assays are made to identify auxotrophic mutants.

...End of the Mutation In E Coli Bacterial Cells...

Please Refer to Biol 368 lab manual* . All procedures are performed according to the BIOL 368 lab manual (Concordia Biology Department 2010) except the followings: in part A, step 6, we added 1.0 ml of Tryptone broth containing sodium citrate to each tube instead of 1.8 ml. Also, for the section data of part B, given data is used instead of our actual section data.

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