...Start of the Role of E6AP in Malignancies and Tumorigenesis...
Leukemia is a malignant hematological disorder characterized by proliferation of abnormal white cells that infiltrate the bone marrow, peripheral blood and other important organs. Leukemia arising from myeloid cells is known as Myeloid Leukemia which may either be chronic myeloid leukemia (CML) and/or acute myeloid leukemia (AML). AML is a complex disease caused by mutations, deregulated gene expression and epigenetic modifications leading to increased proliferation and decreased differentiation of hematopoietic progenitor cells. Several important molecular markers have been discovered in AML to better characterize patients. C/EBPα is an important regulator of Granulopoiesis.
...Middle of the Role of E6AP in Malignancies and Tumorigenesis...
The RING domain provides a docking site for the E2 enzyme, which mediate transfer of ubiquitin to the substrate, facilitating assembly of mono- or polyubiquitylated conjugates via different lysine residues of ubiquitin. The resulting modifications have a diverse range of biological functions, from proteasome-dependent proteolysis (Lys48- and Lys 11-linked polyubiquitin) to post-translational regulation of protein function, structure, assembly, and/or localization (Lys 63 and other linkages)[1, 6]. E3 ligases can also be classified into single subunit E3s (e.g. Mdm2, Cbl) and multi-subunit complexes (APC, SCF). E3 enzymes bind their target substrates through various protein-protein interaction domains (e.g.
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Due to the above relevance and role of E6AP in malignancies and tumorigenesis .. . 1) Expression of GST and GST-E6AP plasmids in BL21 strain of . 2) Purification of GST and GST-E6AP proteins from BL21 strain of . 3) Validation of expression through western blotting . 4) To detect GST-E6AP protein interaction with whole cell lysates of HL-60 cells treated with 1μM ATRA for 0, 24 and 48 hrs .
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Hence, the linkages play an important role in determining the specificity of the ubiquitin function. NF-κB plays an important role as a transcription factor, whose function is inhibited by the IκBα family of inhibit proteins.
RX498 mutants display an abnormal pattern of diffusion, and nuclear and cytosolic localisations, suggesting that in C-termini of the F-Box Only 7 proteins play an important role in nuclear transport. Furthermore, besides cell cycle regulation, CRLs have been implicated to play a role in cellular processes such as the, DNA damage responses, oxidative...
Following the TAD is a proline-rich region which plays an important role in apoptotic activities. The different domains of p53 protein have their functions respectively, nevertheless, they constitute an inseparable entirety to play the role as a tumor suppressor.
Further research has identified the low-molecular-weight compound PRIMA-1 to be a possible way to reactivate apoptosis by p53 to massively cancerous cell. It is composed of 393 amino acids and has four main binding domains: the N terminus (1-43), the beginning of the chain that regulates gene expression, the DNA binding domain (110-286) that is asso...
The MDM2 gene plays a key role in the p53 pathway, and the SNP 309T/G single-nucleotide polymorphism in the promoter region of MDM2 has been shown to be associated with increased risk of cancer. Many studies have demonstrated that IL8 may play a vital role in tumorigenesis.
In a remarkable contrast to the “direct signaling” model, a recent study have showed that O6MeG induce ATM and ATR activation, and inhibition of ATM and ATR sensitizes the cell to monofunctional alkylating agent with more rule to ATR (Eich et al., 2013). This model has been supported by separation of function mutations in mice containing mutations i...
The proteins produced by these oncogenes interact with p53 and pRB proteins causes cell cycle to be interrupted (Boyer, Wazer, & Band, 1996; Huibregtse, Scheffner, & Howley, 1991). Src-homology 2 (SH2) domain play an important role for the activation of STAT protein by binding to phosphptyrosine.
Increase in the level of p53 protein leads to increased protein phosphorylation, acetylation, and other post translational modifications. Activated p53 proteins can: i) stimulate transcription of p21 protein (which arrests progression from G -S checkpoint of mitotic cycle), ii) regulate gene expression that retard replication of DNA (this helps in ...
Antibody is attached to solid support after this step. Continue the incubation .
Ploegh, Amon, and Scott. Molecular Cell Biology, 7th Edition, 2013, Lodish, Berk, Kaiser, Krieger, Bretscher.
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